Document Details
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Document Type |
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Thesis |
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Document Title |
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Synthesis, Characterization, Molecular Docking Study and Application of Some Metal-based Drug Complexes تحضير وتوصيف ودراسة الالتحام الجزيئي وتطبيقات لبعض معقدات الأدوية الفلزية |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Metal ions have played key roles as components of pharmaceuticals in the field of anticancer therapy, arthritis, and cardiovascular medicine. Transition metal ions are well known cancer treatment complexes as cisplatin, which is utilized in nearly 50% of all cancer therapies, despite its significant toxic side effects. Therefore, oncologists have now focused on developing new complexes with less toxicity and which inhibit cancer cell growth by different mechanisms of action when compared with platinum-based therapeutics.
Metal based drugs complexes have drawn attention from the scientific community because of their superior clinical applications compared to the free drugs. For example, zinc sulfadiazine and Ag(I) sulfadiazine are more effective than sulfadiazine and used to promote wound healing and to control infection. Thus, searching for novel metal-based drug complexes is a high priority for medicinal biochemists.
Four acknowledged drugs with several potential binding sites that could be coordinated with metal ions namely, Carbamizole (CMZ), 6-mercaptopurine (6-MP), Sulfaclozine (SCZ) and Thiamphenicol (TM) have reacted with transition metal ions to synthesize their metal complexes. The isolated, highly stable and colored metal complexes confirmed their structures by varied characterization techniques such as elemental analysis, spectral, thermal techniques. Molar ratio and Infrared (IR) spectra are practical method to investigate binding system and atoms that associated the coordination sphere. Moreover, electronic paramagnetic resonance (EPR) spectroscopy is a powerful approach to study the ligand coordination of Cu(II) compounds. The geometry and the color of the newly metal complexes could be confirmed and interpret by the absorption spectroscopic due to the unique transition of the metal ion in solution. Thermogravimetric analysis (TGA) is an essential tool that has been used to study compound stability and lattice water molecules, and to characterize the structure.
Experimentally, various techniques were carried out to investigate the possible influence of the metal ions coordination on their potential as therapeutics. One of the least expensive and simplest methods is spectroscopic titration experiments with CT-DNA to study binding affinity of the metal complexes with the pharmacological target. A molecular docking approach was also used to examine the molecular interaction of the newly synthesized compounds and the free ligand to test their inhibitory capacity towards different cancer protein receptors. Finally, in-vitro cytotoxicity assays using two cell lines, breast cancer cell line (MCF-7) and colon cancer cell line (CaCo-2), were used to evaluate all compounds. |
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Supervisor |
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Dr. Mostafa Ali Hussein |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1443 AH
2022 AD |
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Added Date |
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Sunday, January 22, 2023 |
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Researchers
| عبير علي شرف الدين | sharfalddin, Abeer Ali | Researcher | Doctorate | |
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