Document Details

Document Type : Thesis 
Document Title :
EXPRESSION OF TIE2 (ANGIOPOIETIN RECEPTOR) ON MONOCYTE SUBPOPULATIONS FROM ISCHEMIC STROKE PATIENTS
ظهور مستقبلات الانجيبيوتين على مجموعات خلايا الوحيدات الدموية من مرضى السكتة الدماغية الاقفارية
 
Subject : Faculty of Applied Medical Sciences 
Document Language : Arabic 
Abstract : Expression of Tie2 (angiopoietin receptor) on monocyte subpopulations from ischemic stroke patients Student:Abdullah Ali H Hamami Advisor: Dr. Talal Hussein Qadah Abstract A stroke is characterized by a rapid development of focal clinical signs that indicate a loss of brain function, or causing death of the patient, with unclear causes rather than of vascular source. Different monocytes subpopulations have a distinct role as phagocytic subsets but also act as pro-inflammatory and pro-angiogenic subsets. Recruitment of the monocyte-derived macrophages to the affected brain occurs most abundantly between 3–7 days after the occurrence of ischemic stroke. The current project aimed to investigate the expression levels of Tie2 (a receptor of angiopoietin) on monocytes and their subpopulations from ischemic stroke patients and compare it to control group. Ischemic stroke patients were recruited after diagnosis was confirmed by medical consultants within 2 days of onset. Healthy volunteers matched with age and sex participated as the control group. Fresh blood samples were collected and analyzed by flow cytometry for total monocytes, monocyte subpopulations and Tie2 expressing monocytes , following staining with CD45, CD14, CD16, and Tie2 monoclonal antibodies. Compared to the control group, ischemic stroke patients exhibited an increased proportion of intermediate monocytes CD14highCD16+ (P<0.001) with a low proportion of non- monocytes (P<0.0251). In addition, classical monocyte with CD14highCD16- showed insignificant difference between patients and control (P<0.489). Furthermore, Tie2 expressing monocytes were significantly higher in ischemic stroke patients compared to the control group (P<0.001). In conclusion, our results demonstrated dysregulation of the angiogenic function of monocytes subsets, which could be an early diagnostic marker to start eithera neurovascular repair therapy and improve medication attributed to highly senescent subpopulations. 
Supervisor : Dr. Talal Hussein Qadah 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Dr. Azizah Rashed Alrafiah 
Added Date : Monday, November 25, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
عبدالله علي هماميHamami, Abdullah AliResearcherMaster 

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