Document Details

Document Type : Thesis 
Document Title :
Potential Effects of Phoenix dactylifera (Ajwa) Extract on Prompting Apoptosis in Human Hepatocellular Carcinoma Cell Line, HepG2
التأثير المحتمل لمستخلص العجوة (Phoenix dactylifera) على إحداث موت مبرمج لخلايا سرطان الكبد البشريةHepG2
 
Subject : Faculty of Science 
Document Language : Arabic 
Abstract : Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide, accounting for 9.1% of all cancers and an estimated incidence of 746,000 new cases every year. Globally, HCC is three times more common in men than in women. It is also one of the leading causes of cancer related deaths in the Kingdom of Saudi Arabia (KSA). The overwhelming majority of HCC cases occurs in patients with chronic liver disease. So that exploration of novel therapeutic modalities is urgently imperative. The present study was undertaken to investigate the inhibitory effects of extract prepared from medicinal plants, Phoenix dactylifera (PDE) on proliferation of HCC HepG2 cells and to elucidate the mechanisms of action. To achieve these aims, we utilized MTT, clonogenic, wound healing (cellular migration), comet and DNA fragmentation assays. Morphological features of apoptosis were detected by Giemsa stain and biochemical changes related to of apoptotic cell death were monitored by assaying both of mitochondrial membrane potential and generation of reactive oxygen species (ROS) using fluorescent dyes, Jc-1 and 2,7 -dichlorofluorescein diacetate (DCFH-DA), respectively. We found that PDE obviously suppressed, in a dose- and time-dependent manner, the proliferation, clonogenicity and cellular migration potentials of the HepG2 cells. Hallmarks of morphological and biochemical signs of apoptosis were clearly apparent in the cells treated with the PDE. The comet assay and agarose gel electrophoresis confirmed that PDE induced DNA damage and oligo-nucleosomal degradation (DNA laddering), respectively in the treated cells. PDE treatment also induced oxidative stress characterized by an increase in the intracellular of ROS and a decrease in the mitochondrial membrane potential. These results strongly suggest that PDE has a potential to inhibit growth and proliferation of HepG2 cells through prompting apoptotic mechanisms. 
Supervisor : Dr. Rania Marwan Makki 
Thesis Type : Master Thesis 
Publishing Year : 1441 AH
2019 AD 
Co-Supervisor : Prof. Ayman Ibrahim A. Elkady 
Added Date : Thursday, September 19, 2019 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
لما محمد القرشيAl-qurashi, Lama MohammedResearcherMaster 

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