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Document Type |
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Thesis |
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Document Title |
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Investigation of prognostic biomarkers in ovarian cancer by using immunohistochemistry technique استكشاف المؤشرات الحيوية الاستشرافية لسرطان المبيض باستخدام تقنية الصبغ المناعي الكيميائي |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Background: The extracellular matrix (ECM) provide the cell suitable conditions for proliferation, migration and/or death. Disruption of ECM is important for tumor cells to proliferate, detach and invade other tissues. Cell adhesion molecules are existing on the surface of epithelial cells to assist them stick to ECM. Claudins are major cell adhesion molecules working at tight junctions. While matrix metalloproteinases (MMPs) caused degradation of ECM, TIMP metallopeptidase inhibitor (TIMPs) inhibit their proteolytic activity. The aim of study was to assess the association of N-cadherin, claudin6 (CLDN6), MMP1 and TIMP3 proteins expression profiles with the ovarian cancer (OC) clinico-pathological features and survival outcomes.
Patients and Methods: 170 of OC FFPE samples were collected from King Abdulaziz University Hospital, Saudi Arabia and constructed in tissue microarray slides. Automated Ventana immunostainer platform was used to evaluate their expression patterns in OC.
Results: Our results showed, significant correlations of N-cadherin with grade (p= 0.05) and subtype (p= 0.05), CLDN6 with size (p= 0.04), stage (p= 0.05) and recurrence (p= 0.009), MMP1 with subtype (p= 0.04), metastasis (p= 0.04) and endpoint status (p= 0.004), and TIMP3 with stage (p= 0.05). The Kaplan-Meier analysis of disease free survival (DFS) outcomes of OC patients showed a significant difference (p= 0.01) between patients with high expression of cytoplasmic CLDN-6 compared with those with low expression, and a significant correlation (p= 0.025) in disease specific survival (DSS) between patients with high TIMP3 expression who were shown to live significantly longer compared with those with lower TIMP3 expression.
Conclusion: Our results showed that CLDN6 is suitable OC prognosticator of DFS, while TIMP3 was associated with advanced stages of OC and DSS. However, additional studies, using more markers and large cohorts to demystify the molecular complexity of OC and alleviate the burden of this deadly malignancy in Saudi women are recommended. |
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Supervisor |
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Prof. Adeel Chaudhary |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Dr. Mourad Assidi |
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Added Date |
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Wednesday, July 31, 2019 |
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Researchers
| سحر سامي حكمي | Hakamy, Sahar Sami | Researcher | Master | |
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