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Document Type |
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Thesis |
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Document Title |
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Effect of heat shock on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs): Implications in regenerative medicine تأثير الحرارة على قدرة الخلايا الجذعية على الانقسام والتحول إلى خلايا غضروفية لمرضى تآكل الغضاريف |
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Subject |
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Faculty of Applied Medical Sciences |
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Document Language |
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Arabic |
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Abstract |
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Autologous stem cells transplantation for articular cartilage repair appears promising. However, increase or decrease in temperatures as associated with the use of arthroscope or laser drilling during stem cell based cartilage repair procedures may have detrimental effects on the transplanted stem cells (Steadman JR et al, 2007). In the present study, we attempt to evaluate the effect of heat shock on human bone marrow mesenchymal stem cells (hBM-MSCs) in relation to its proliferation, survival and gene expression of chondrogenic differentiation.
Primary cultures of hBM-MSCs were established and characterized. Early passages of hBM-MSCs (20x103cells) were exposed independently to arthroscope generated heat and also direct heat at different temperatures (37˚C, 40˚C, 50˚C, 55˚C) and duration (30s, 60s, 90s, 120s) either as cell- pellet or as cell suspensions. Following heat shock the hBM-MSCs were cultured under standard culture conditions for 24h and changes in morphology (Phase-contrast imaging), cell proliferation (MTT assay), cell apoptosis (AnnexinV-FITC and PI) and cartilage related gene expression (qRT-PCR) were evaluated.
Exposure to heat shock affected the cellular functions which was more pronounced in the suspension cells than pelleted cells. Cell death were observed in both groups at elevated temperatures (55˚C) and longer durations (90s, 120s). There was an overall mild increase in cell proliferation in both cell-suspension and cell-pellet groups at 24h at lower temperatures and shorter duration. However, there was nearly 50% decrease in inhibition in the cell-suspension group compared to the cell-pellet group at higher temperatures and duration. Significant changes were observed in the cell proliferation and cartilage related genes in the cell-pellet group compared to the cell-suspension group.
The present study identified that cell-pellet have better proliferation, survival and chondrogenic differentiation compared to cell-suspension in response to heat shock and thus may be useful to transplant cells as pellet form for regenerative medicine applications. |
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Supervisor |
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Prof. Mamdooh Gari |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1438 AH
2017 AD |
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Co-Supervisor |
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Dr. Kalamegam Gauthaman |
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Added Date |
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Tuesday, September 26, 2017 |
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Researchers
| رهام ناهض النونو | Al-Nono, Reham Nahedh | Researcher | Master | |
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